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IJSTR >> Volume 8 - Issue 11, November 2019 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

A study on the antibacterial activity in the haemolymph of larvae of the mulberry silkworm, bombyx mori l. In response to injection of the bacteria, bacillus cereus

[Full Text]



Ponmurugan, M. Karthikeyan, A



Zone of inhibition, Haemolymph, Antibacterial acitivity, Attenuated bacteria



The antibacterial activity of haemolymph of the induced fifth instar larvae of the mulberry silkworm, Bombyx mori in response to Bacillus cereus was investigated by well diffusion method. The control larvae C1 were fed with normal leaves till cocoon spinning. The second set of control larvae C2 were injected with bacteria broth solution. The experimental larvae E1 were injected with live bacteria B. cereus, E2 were injected with attenuated bacteria, E3 were injected with a mixture of haemolymph derived from E1 larvae and live bacteria and E4 were injected with a mixture of haemolymph derived from E2 larvae and live bacteria. Zone of inhibition for the haemolymph of E1 and E2 against E. coli was 6mm and 5mm respectively. Likewise zone of inhibition of E1 and E2 was 7mm and 5mm respectively against B. cereus. Only minimum zone of inhibition of 2mm and 1mm was observed against both Staphylococcus aureus and Klebsiella pneumonia respectively. It was inferred that Escherichia coli and B. cereus were sensitive to haemolymph of E1 and E2. Purified haemolymph proteins exhibited a moderate zone of inhibition of 3mm against all the four bacteria tested. Compared to crude haemolymph purified sample of E2 showed greater antibacterial effect against Staphylococcus aureus and Klebsiella pneumonia.



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