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IJSTR >> Volume 8 - Issue 11, November 2019 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Comparative Study Of Growth And Sporulation Of Magnaporthe Oryzae Using Different Media Formulations.

[Full Text]



Kalpana Kulkarni, Dr. Swati Peshwe



Blast disease, growth, KKSP medium, Magnaporthe oryzae, mycelia, melanisation, sporulation



Growth and Sporulation of Magnaporthe oryzae isolate has been studied by different people across world since screening of rice varieties for Blast disease resistance requires large number of inoculum preparation. Fifteen different natural and synthetic media were studied to understand the nutritional requirements of the blast pathogen–naming few Oat meal agar, rice media, rice and soyabean extract media. Mycelial yield, number of spores, radial growth were observed and compared. Based on the requirements of Magnaporthe oryzae, a new medium named KKSP formulated medium was composed for growth and sporulation studies. It was found that the formulated medium was also suitable medium for the growth of blast pathogen, where high mycelium yield was achieved in shorter time as compared with other standard media used so far. The KKSP formulated medium showed pink pigmentation and newly formed mycelia could be easily identified with morphology and septation pattern with visual observation.



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