IJSTR

International Journal of Scientific & Technology Research

IJSTR@Facebook IJSTR@Twitter IJSTR@Linkedin
Home About Us Scope Editorial Board Blog/Latest News Contact Us
CALL FOR PAPERS
AUTHORS
DOWNLOADS
CONTACT
QR CODE
IJSTR-QR Code

IJSTR >> Volume 2- Issue 12, December 2013 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Evaluation Of Toxicological Effects Of Carbon Tetrachloride On Liver Enzymes Of Wistar Rats

[Full Text]

 

AUTHOR(S)

Ezejindu DN, Ulolene G.C, Ihentuge C.J

 

KEYWORDS

Key words: Liver enzymes, Carbon tetrachloride, Liver weight, Body weight, Wistar rats.

 

ABSTRACT

ABSTRACT: This work is aimed at determining the effects of carbon tetrachloride on liver enzymes of adult wistar rats following oral administration. Twenty adult wistar rats weighing between 190-220kg were used. They were allocated into four groups (A,B,C &D) of five animals each. Group A animals served as the control and received 0.5ml of distilled water. The experimental groups B,C & D received 0.1ml, 0.2ml and 0.3ml of carbon tetrachloride respectively for 21 days. Twenty four hours after the last administration, the animals were weighed, anaesthetized under chloroform vapour and dissected. The liver tissues were removed and weighed. Blood samples were collected by cardiac puncture using sterile syringes and needles. Blood for serum preparation was collected into sterile plain tubes without anti-coagulant. Serum samples were separated into sterile plain tubes and stored in the refrigerator for analysis. The activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were determined using randox kit method. The final body weight of the experimental groups were significantly lower (P<0.001) than the control (A). the levels of mean aspartate aminotransferase, alatine aminotransferase and alkaline phosphatase in group D were significantly higher (P<0.001) than groups B,C and control A. The levels of mean aspartate aminotransferase, alanine aminotransferase and alkaline phosphotase in group C were significantly higher (P<0.001) than group B and control A. The result from this study showed that adverse biochemical changes are associated with animals in groups B,C and D. The present study indicated dose-dependent on the liver enzymes of rats administered with CCL4. Our findings therefore suggest that chronic CCL4 consumption may put the liver at risk of adverse biochemical alterations and histopathological lesions.

 

REFERENCES

[1]. Agency for Toxic substances and disease registry (ATSDR) “Toxicological profiles for carbon tetranscholoride” (update), Atlanta G.A Us Department of Health and Human Services, Public Health services, 2005.

[2]. Cameroon GR, Karunaratne W.A.E, Thomas JC “Massive necrosis (toxic infeaction) of the liver following intra-portal administration of poisons” J.Path. Bact 44, 297-303, 1983.

[3]. Aterman K “Studies in fibrosis of the liver induced by carbon tetrachloride (relative between hepatocellular injury and new formation of fibrosis tissue)” AMA. Arch Path 57, 1 – 11, 1954.

[4]. Seifert WF, Bosma A, Brouwer A et al “Vitamin A deficiency potentiates carbon tetrachloride induced liver fibrosis in rats”, Hepatology 19 (1) : 193 – 201, 1994.

[5]. Liu KX, Kato Y, Yamaxak M, Higuchi O, Nakamura T, sugiyama “Decrease in the hepatic clearance of hepatocyte growth factor in carbon tetrachloride intoxicated rats” Hepatology 17 (4): 651 – 60, 1993.

[6]. Recknagel RO, Glende EA, Dolak JA, waller RL “Mechanism of carbon tetrachloride toxicity”, Pharmatology therapeutics 43 (43): 139 – 154, 1989.

[7]. US Environmental Protection Agency “Carbon tetrachloride Health advisory office of drinking water”, Washington DC, 1987.

[8]. US Department of Health and Human services Hazardous substances Databank “National Toxicology Information Program, National Toxicology Information Program”, National Library of Medicine, Bethesda MD,1993.

[9]. Goodley Eugene L, Diagnostic “Enzymology submit query lea and febiger publications”, ISBN- 081210044, 1970

[10]. Wright PJ and DJ Plummer “The use of urinary enzyme measurements to detect renal changes caused by nephrotoxic compounds”, Biochem pharmacol. 12: 65-68, 1974.

[11]. Christian P and D.E Metzler “Transminases”, ISBN10: 0471085014, 1985.

[12]. Cotran R, Kumar V and Robins S Robins “Pathological basis of disease” 4th edn. WB Saunders Co. Harcourt Pp: 212 – 217, 1989.

[13]. Ngaha EO “Renal effects of potassium dichromate in the rat: composition of urinary excretion with corresponding tissue pattern”, Gen. Pharmacol 12: 291 – 358, 1981.

[14]. Moss D.W and Rosalki, SB “Enzyme Tests in Diagnosis”, 2nd Edition, Edward Amold, London Pp 2012 – 213, 1996.