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IJSTR >> Volume 3- Issue 1, January 2014 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Seroprevalence Of Visceral Leishmaniasis Using Direct Agglutination Test (DAT) In Tabark Allah Village, Eastern Sudan, 2010.

[Full Text]



Mohammed A Elawad, Kamil M Ali, Abdelsafi A gabbad, Omran F Osman, Ahmed M Musa



Key words: direct agglutination, donovani, endemic, leishmaniasis, seroprevalence, visceral,



Absract: Visceral Leishmaniasis (VL) is a protozoan disease caused by Leishmania donovani and transmitted by phlebotomus sand fly. The disease is endemic in Sudan and large area of tropics. It has been reported in eastern Sudan since the beginning of the twentieth century. This is a cross-sectional study conducted in Tabark Allah Village, eastern Sudan to measure the seroprevalence of visceral Leishmaniasis. Samples of blood sera were collected from 373 individuals selected by systematic random technique. The samples were examined using Direct Agglutination Test (DAT) to detect the anti-leishmania antibodies. Demographic and socio-economic data were obtained by direct interview with the study group using a pre-tested questionnaire.The seroprevalence at a cut off ≥ reciprocal titre of 3200 was found to be 17.2% which was more prevalent among children (70.2%) with p value of 0.000, compared to adults. Family income had a significant association with the seroprevalence of visceral Leishmaniasis (p value: 0.05).



[1]. Aeesha N J, Lawrence J, Anthony D M, and Diana N J. Changing pattern of visceral leishmaniasis, United Kingdom, 1985-2004. Emerging Infectious Diseases 2006; 12(8): 1257-59.

[2]. Bahador S , Chance M, and Hommel M. A capture ELIZA for the diagnosis of visceral leishmaniasis using a monoclonal antibody against a leishmanial urinary antigen. Indian Biomedical Journal 2005; 9(3): 117-122.

[3]. Bhattacharya S K, Dipika S, & Juntra K. Childhood visceralleishmaniasis. Indian J Med Res 2006; 123: 353-356.

[4]. Camargo L B, and Langoni H. Impact of leishmaniasis on public health. J Venom Anim Toxins incl Trop 2006; 12(4): 527-548.

[5]. Caryn Bern, Allen W. Hightower, Rajib Chowdhury, Mustakim Ali, Josef Amann, Yukiko Wagatsuma, Rashidul Haque, Katie Kurkjian, Louise E. Vaz, Moarrita Begum, Tangin Akter, Catherine B. Cetre-Sossah, Indu B. Ahluwalia, Ellen Dotson, W. Evan Secor, Robert F. Breiman, and James H. Maguire. Risk Factors for Kala-Azar in Bangladesh. Emerging infectious diseases 2005; 11(5): 655-662.

[6]. Caren Bern, Courtenay O, Alvar J. Of Cattle, Sand Flies and Men: A Systematic Review of Risk Factor Analyses for South Asian Visceral Leishmaniasis and Implications for Elimination. PLoS Negl Trop Dis 2010; 4(2): e599. doi:10.1371/journal.pntd.0000599

[7]. Dia- el – Din A E, Judith S, Anna B, Valerie O, Maha E O, Abdelrafie M M, Stephen J C, Richard W A, Madeleine C T. Risk mapping of visceral leishmaniasis: The role of local variation in rainfall and altitude on the presence and incidence of kala-azar in eastern Sudan. AMJ Trop Med Hyg 2003; 68(1): 10-17.

[8]. Dilvia F S and Simao D V. A ten year (1990-1999) survey on leishmaniasis incidence in Pernambuco state, northeastern Brazil. Revista De Patologia Tropical 2003; 32(1): 53-62.

[9]. Eduardo S S, Gerard J S, Celia M F, Reginaldo P B, Raquel S P, & Henk D F. Application of Direct Agglutination Test (DAT) and Fast Agglutination Screening Test (FAST) for sero-diagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil. Kinetoplastid Biology and Disease 2005; 4(4): doi: 10.1186/1475-9292-4-4.

[10]. Elnaiem D E, Mukhawi A M, Hassan A A, Osman M E, Osman O F, Abdeen M S, & Abdel Raheem M A. Factors affecting variations in exposure to infections by Leishmania donovani in eastern Sudan. Eastern Mediterranean Health Journal 2003; 9(4): 827-36.

[11]. El-Safi S H, Bucheton B, Kheir M M, Musa H A, EL-Obaid M, Hammad A, and Dessein A. Epidemiology of visceral leishmaniasis in Atbara River area, eastern Sudan: the outbreak of Barbar El-Fugra village (1996-1997). Trop Med Int Health 2002; 9(12): 1305-11.

[12]. El-Safi S H, Hamid N, Omer A, Abdel-Haleem A, Hammad A, Kareem H G, and Boelaert M. Infection rates with Leishmania donovani and Mycobacterium tuberculosis in a village in eastern Sudan. Trop Med Int Health 2004; 9(12): 1305-11

[13]. Farzin-Nia B, and Hanafi-Bojd A A. The sand fly fauna of an endemic focus of visceral leishmaniasis in central Iran. Iranian J Arthropod-Borne Dis 2007; 1(2): 48-52.

[14]. Indu B. Ahluwalia, Caryn Bern, Cristiane Costa, Tangin Akter, Rajib Chowdhury, Mustakim Ali, Didarul Alam, Eben Kenah, Josef Amann, Meghla Islam, Yukiko Wagatsuma, Rashidul Haque, Robert F. Breiman, And James H. Maguire. Visceral Leishmaniasis: Consequences Of A Neglected Disease In A Bangladeshi Community. Am. J. Trop. Med. Hyg 2003; 69(6), pp. 624–628.

[15]. Israel C, Chicharro C, Nieto J, Bailo B, CanavateC, Fgueras M, and Alvar J. Comparison of new diagnostic tool for management of pediatric Mediterranean visceral leishmaniasis. Journal of Clinical Microbiology 2006; 44(7): 2343-2347.

[16]. Khlabus. Kh. Raddam. Clinical and epidemiological features of kala-azar in Thi-Qar Governorate. MJBU 2007; 25(1): 51-54.

[17]. Koert R, Yoseph M, Marius M, Sammy K, Caroline O, & Robert N D. Evaluation of a new recombinant k39 rabid diagnostic test for Sudanese visceral leishmaniasis. Am J Trop Med Hyg 2006; 74(1): 76-80.

[18]. Koirala S, Karki P, Das M L, Parija S C, & Karki B M. Epidemiological study of kala-azar by direct agglutination test in two rural communities of eastern Nepal. Trop Med Int Health 2004; 9(4): 533-7.

[19]. Kordofani Y M, Nour Y M, El-Hassan A M, and Shalayel M H. Post kala-azar dermal leishmaniasis in Sudan. Eastern Mediterranean Health Journal 2001; 7(6): 1056-1060

[20]. Mo'awia M Hassan, Omran F Osman, Fathi MA El-Raba'a, Henk DFH Schallig and Dia-Eldin A Elnaiem. Role of the domestic dog as a reservoir host of Leishmania donovani in eastern Sudan. Parasites & Vectors BioMed Central 2009; 2:26 doi:10.1186/1756-3305-2-26

[21]. Muhammad Uzair, Sheraz Jamal Khan, Syed Munib, Fazal Raheem and Syed Humayun Shah. Visceral Leishmaniasis (Kala Azar): Presentation, Diagnosis And Response To Therapy (An Experience Of Ten Cases In Adults). Gomal Journal of Medical Sciences 2004; 2(1). 9-12.

[22]. Olga Z, Marian U, Rafael B, Vestalia R, Marta C, Emilia N, Doris B, & Jacinto C. Epidemiological aspects of human and canine visceral leishmaniasis in Venezuela. Rev Panam Salud Publica/Pan Am J Public Health 2003; 13(4): 239-45.

[23]. Sarker C B, Chowdhury K S, Siddiqui N I, Jamal M F, Rahman S, Momen A, Dhar D K, & Alam K S. Clinical profile of kala-azar in adults: as seen in Mymensingh MedicalCollege Hospital, Mymensingh, Bangladesh. Mymensingh Med J 2003; 12(1): 41-44.

[24]. Sarman S. New development in diagnosis of leishmaniasis. Indian J Med Res 2006; 123: 311-330.

[25]. Singh SP, Picado A, Boelaert M, Gidwani K, Andersen EW, Ostyn B, Meheus F, Rai M, Chappuis F, Davies C, Sundar S. The epidemiology of Leishmania donovani infection in high transmission foci in India. Trop Med Int Health.15 Suppl 2010; 2:12-20.

[26]. Zélia M. P. Luz Mariângela Carneiro Virgínia Schall Ana Rabello. The organization of health services and visceral leishmaniasis: an integrated intervention to improve diagnosis and treatment Cad. Saúde Pública, Rio de Janeiro 2009; 25(5):1177-1184.

[27]. Zijlstra E E and el-Hassan A M. Leishmaniasis in Sudan: visceral leishmaniasis. Trans R Soc Trop Med Hyg 95 suppl 2001; 1: s27-58