International Journal of Scientific & Technology Research

IJSTR@Facebook IJSTR@Twitter IJSTR@Linkedin
Home About Us Scope Editorial Board Blog/Latest News Contact Us

IJSTR >> Volume 4 - Issue 12, December 2015 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Antagonistic Activity Of Endophytic Bacteria Isolated From Mentha Rotundifolia L.

[Full Text]



Elhartiti Abla, Elhabchi Souad, Hichar Abdelhadi, Omar Bazdi, Ounine Khadija



Keywords: Bacteria endophytic, Bacillaceae, Pseudomonadaceae, antagonistic activity, Fusarium oxysporum, Aspergillus niger, Botrytis cinerea



Abstract: This study is implemented for the isolation, purification and identification of endophytic bacteria which produces antifungal substances from the roots of Mentha rotundifolia L. The 59 obtained bacterial isolates were tested for their antagonistic activity by the dual confrontation against the phytopathogenic fungi Fusarium oxysporum, Aspergillus Niger and Botrytis cinerea. Eight bacterial strains were selected for their strong antifungal activity. These are strains M21, M23, M3a, M4, M14d and M3c which belong to the family Bacillaceae, M12 and M3b which belongs to the family of Pseudomonadaceae. Among these, three bacterial strains namely M21, M23 and M12 induce 70% of inhibition of mycelial growth of phytopathogenic fungi Fusarium oxysporum and Aspergillus Niger while the five bacterial strains M3a, M3c, M3b, M4 and M14d have proved to be effective in inhibiting more than 60% of mycelial growth of Botrytis cinerea.



[1] Rosslenbroich H. J. et Stuebler D. 2000.Botrytis cinerea – history of chemical control and novel fungicides for its management. Crop Prot., 19, 557-561.

[2] Gudelj I., Fitt B. D. L. et van den Bosch F. 2004. Evolution of sibling fungal plant pathogens in relation to host specialization, Phytopathol., 94, 789-795.

[3] Hubert J., Stejskal V., Munzbergova Z., Kubatova A., Vanova M. et Zdarkova E. 2005. Mites and fungi in heavily infested stores in the Czech Republic, J. Econ. Entomol., 97, 2144-2153.

[4] Lin L, Qiao YS, Ju ZY, Ma CW, Liu YH, Zhou YJ, Dong HS 2009. Isolation and characterization of endophytic Bacillus subtilis Jaas ed1 antagonist of eggplant Verticillium wilt. Biosci Biotechnol Biochem 73:1489-1493.

[5] Moenne.Loccoz., Y., Powell, J., Higgins, P., McCarthy, J., O´Gara, F.1998. An investigation of the impact of biocontrol Pseudomonas Fluorescens F113 on the growth of sugar beet the performance of subsequent clover-Rhizobium symbiosis. Appl.Soil.Ecol. 7, pp.225-237.

[6] Zhao LF, Deng ZS, Yang WQ, Cao Y, Wang ET, Wei GH (2010). Diverse rhizobia associated with Sophora alopecuroides grown in different regions of Loess Plateau in China. Syst Appl Microbiol 33:468-477.

[7] Wang S.L., Hsaiao W.J., Chang W.T. et al., 2002. Purification and characterization of an antimicrobial chitinase extracellularly produces by monoscus purpureus CCR 31499 in a shrimp and crab shell powder medium. J.Aric.Food.Chem., 50: 2249-2255

[8] Bergey´s Manual of Systematic Bacteriology, 2001, second ed.

[9] Nourozian, J., H.R. Etebarian and G. Khodakaramian, 2006. Biological control of Fusarium graminearum on wheat by antagonistic bacteria, Songklanakarin J. 19. Sci. and Technol., 28: 29-38.

[10] Munimbazi, C. and L.B. Bullerman, 1998. Isolation and partial characterization of antifungal metabolites of Bacillus pumilus. J. Appl. Microbiol., 84: 959-968.

[11] Sadfi-Zouaoui N., Essghaier B., Hannachi I., Hajlaoui M.R and Boudabous A. 2007. First report on the use of moderately halophilic bacteria against stem canker of greenhouse tomatoes caused by Botrytis cinerea. Ann. Microbiol. 57(3) : 337-339.

[12] Toure, Y., Ongene, M., Jacques, P.,Guiro, A., Thonart, P., 2004. Role of lipopeptides produced by Bacillus subtilis GA1 in the reduction of grey mould disease caused by botrytis cinerea on apple. Journal of Applied Microbiology 96, 1151-1160.

[13] Jeffrey, D., L. Palumbo, James, Baker, E. Noreen and Mahoney, 2006. Isolation of Bacterial Antagonists of Aspergillus flavus from Almonds, Microbial Ecol., 52(1): 45-52.

[14] Palumbo, J.D., T.L. O’Keeffe and H.K. Abbas, 2007. Isolation of maize soil and rhizosphere bacteria with antagonistic activity Aspergillus flavus and Fusarium verticillioides, J. Food Protection, 70: 1615-1621.

[15] Srivastava, R. and Shalni, 2008. Antifungal Activity 8. Pseudomonas fluorescence Against Different Plant Pathogenic Fungi, Electronic J. Environmental, Agriculture and Food Chemistry, 7: 2789-2796.

[16] Paez, M., Martienz-Nieto, P., Bernal-Castillo, J., 2005. Siderophore producing Pseudomonas and pathogenic Rhizoctonia solani and Botrytis cinerea antagonists. Univ. Sci. 10, 65-74.