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IJSTR >> Volume 6 - Issue 1, January 2017 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Gene Pool Of The Body Banding Patterns Of Barbus Tetrazona At North Sumatra

[Full Text]



Deny Supriharti, Elimasni



Barbus tetrazona, gene frequency and genotype frequency



Study of gene frequencies and genotype of Sumatra Barbus was conducted to determine the the gene pool of body banding patterns of Sumatra Barbus (Barbus tetrazona) This research were done by examined the genotype and gene frequency in its population at North Sumatra. Sample of Barbus tetrazona were collected among its distributors at Medan and kept at several fish tanks. The acclimatizations were done for several months to growth and mature. When the fish reached its maturity, male and female were separated in a special fish tanks and induced by using a reproductive hormone ( ovaprim 0.5 mg / kg body) for 2 times with an interval 8 hours. Then the male and females of the fish were conducted to mate. After spawning happened, the fry would be separated. The fries were fed by rotifers and maintained until they reach maturity and ready to be mated. The hybrids would be carried out between individuals (P), F1 and F2, also testcross and back cross. Data obtained from those hybrids were analysed by Chi- Square based on Mendel law assumption. The result of the research showed that the majority of Barbus crossing were fit with Mendelís assumption, except 2 crossing showed unfitness from Mendelís assumption. The genotype and gene frequencies of the body banding pattern of Barbus also in line with Mendelís law. The additive complete dominant trait has the highest genotype and gene frequencies which are 0.62 and 0.55 respectively, and the recessive trait also showed the lowest genotype and gene frequencies, 0.09 and 0.29 respectively. Meanwhile the incomplete body banding had the genotype and gene frequencies 0.29 and 0.16 respectively.



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