IJSTR

International Journal of Scientific & Technology Research

IJSTR@Facebook IJSTR@Twitter IJSTR@Linkedin
Home About Us Scope Editorial Board Blog/Latest News Contact Us
CALL FOR PAPERS
AUTHORS
DOWNLOADS
CONTACT
QR CODE
IJSTR-QR Code

IJSTR >> Volume 5 - Issue 12, December 2016 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Isolation Of Total RNA Silkworm (Bombyx Mori L.) Larvae (LEPIDOPTERA:BOMBYCIDAE) C301 On Their Several Body Tissues

[Full Text]

 

AUTHOR(S)

Masitta Tanjung, Maryani Cyccu Tobing, Darma Bakti, Syafruddin Ilyas

 

KEYWORDS

Bombyx mori, cuticle layer, rectum, silk gland

 

ABSTRACT

Bombyx mori (Lepidoptera:Bombycidae) a group of insect has high economy value. Continually domestication by human leads to the insect get loss of its productivity and more sensitive to high temperature especially while its cultivated in tropical climate. The temperature effect HSP gene of Bombyx mori larvae that responsible for maintaining the body from temperature stress. The gene was obtained by isolation total RNA of larval 5th instar from several organs such as head, silk gland, cuticle layer and and rectum using Trizol reagent. Results showed that the total RNA was found on larval head, silk gland and rectum, whereas, clumping of calcium carbonate occurred on cuticle layer. Based on analytical and RNA quantitative quality using Nanospectrophotometer UV-vis indicate that RNA total on larval cuticle was not detected because of undisolved RNA total in DEPC water and ddH2O.

 

REFERENCES

[1] Abdel-Banat, B.M.A and Koga, D. 2016. Alternative Splicing of the Primary Transcript Generates Heterogeneity within the Products of the Gene for Bombyx mori Chitinase. J. Bio. Che. 277(34) : 30524-30534.

[2] Azuma M, Nagae T, Maruyama M, Kataoka N, (2012). Two water-specific aquaporins at the apical and basal plasma membranes of insect epithelia: molecular basis for water recycling through the cryptonephric rectal complex of lepidopteran larvae. J. Insect Physiol. 58: 523-533.

[3] Bergant, K. and Trdan, S. 2006. How reliable are thermal constants for insect development when estimated from laboratory experiments?. Ent. Exp. App.120 : 251-256

[4] Bhattacharjee, S. 2008. Triadimefon pretreatment protects newly assembled membrane system and causes up-regulation of stress proteins in salinity stressed Amaranthus lividus L. during early germination. J. Environ. Biol 29

[5] Borgohain. A. 2015. Silk and its Biosynthesis in Silkworm Bombyx mori. Areview. J. Acad. Indust. Res. 3 (1) : 626-628

[6] Brooks, G. 1998. Biotechnology in Healthcare: An Introduction to Biopharmaceuticals, Pharmaceutical Press, London

[7] Brusca, R. C. and Brusca, G. J. 2003. Taxonomy of invertebrates. Sunderland, Massachusetts

[8] Buckingham, L. and Flaws, M. L. 2007. Molecular Diagnostics: Fundamentals, Methods, & Clinical Applications, F.A. Davis, Philadelphia, Pa, USA

[9] Chapman, R.F. 1998. The Insect Structure and Function. 4th edn. Amer. Elsev, New York

[10] Chomczynski, P. and Sacchi, N. 2006. The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on. Nat. Pro. 1 : 581 - 585

[11] Chen, A.C., Mayer, R. T. and DeLoach, J. R. 1982. Purification and characterization of chitinase from the stable fly, Stomoxys calcitrans. Arch. Biochem. Biophys. 216 (1) : 314-321

[12] Doyle, K. 1996. The Source of Discovery: Protocols and Applications Guide, PROMEGA, Madison, Wis, USA

[13] Gao, K., Deng, X. Y., Shang, M.K., Qian, H.y. and Guo, X. J. 2016. Molecular cloning and characterization of biphenyl hydrolase-like (BPHL) protein gene from silkworm, Bombyx mori. J. Asia-Pac. Ent. 19 (3) : 611-617

[14] Gulrajani, M.L. 1988. Degumming of silk; in Silk dyeing printing and finishing, M.L. Gulrajani (ed), Department of Textile Technology Indian Institute of Technology, New Delhi. pp.63-95

[15] Henson H (1937). The structure and post-embryonic development of Vanessa urticae (Lepidoptera). Quart. J. Micr. Sci. 74: 321-360

[16] Hochachka, P.W. and Somero, G.N. 2002. In: Biochem. Adapt. Oxford University Press, New York

[17] Hong, S.W. and Vierling, E. 2000. Mutants of Arabidopsis thaliana defective in acquisition of tolerance to high temperature stress. Proc. Nat. Acad. Sci. USA, 97: 4392-4397

[18] Hussain, M., Naeem, M., Khan, S.A., Bhatti, M.F., and Munawar, M. 2011. Studies on the influence of temperature and humidity on biological traits of silkworm (Bombyx mori L: Bombycidae) Afr. J. Biotech. 10 (57):12368-12375

[19] Ito, K., Shimura, K., Katsuma, S., Tsuda, Y., Kobayashi, J., Tabunoki, H., Yokoyama, T., Shimada, T. and Kadono-Okuda, K. 2016. Gene expression and localization analysis of Bombyx moribidensovirus and its putative receptor in B. mori midgut. J. Inv. Path, 136 : 50–56

[20] Jakaria, S.; S. Mansjoer; A. Saefuddin dan M. Koamini 2001. Analisis interaksi genotipe-lingkungan pada beberapa sifat kualitatif ulat sutera (Bombyx mori. L. Met. Pet. 24 (2)

[21] Kamili, A. and Masoodi M.A. 2000. Silk and its biosynthesis. Principle of Temperate Sericulture. pp. 86-104.

[22] Kataoka, H., Nagasawa, H., Isogai, A., Ishizaki, H. and Suzuki, A. 1991. Prothoracicotropic Hormone of the Silkworm, Bombyx mori : Amino Acid Sequence and Dimeric Structure. Agricul. Biol. Chem. 55 (1) : 73-86

[23] Khan, M. M. 2014. Effects of Temperature and R.H. % on Commercial Characters of Silkworm (Bombyx mori L.) cocoons in Anantapuramu district of AP, India. Res. J. Agr. Forest. Sci.2(11) :1-3

[24] Koga, D., Funakoshi, T., Mizuki, K. Ide, A., Kramer, K. J., Zen, K. C., Choi. H. and Muthukrishnan, S. 1992. Immunoblot analysis of chitinolytic enzymes in integument and molting fluid of the silkworm, Bombyx mori, and the tobacco hornworm, Manduca sexta. Ins. Bioch. Mol. Bio. 22(4): 305–311

[25] Koga, D., Jilka, J. and Kramer, K. J. 1983. Insect Endochitinases: Glycoproteins From Moulting Fluid, Integument And Pupal Haemolymph Of Manduca Sexta L. Insect. Biochem. 3 (3) : 295-305.

[26] Komatsu, K. 1980. Recent advances in sericin research. J. Sericult. Sci. Japan. 69: 457-465.
[27] Kojima, K. and Ozawa, S. 2002. Method for isolating and purifying nucleic acids. United State patent US.

[28] Kumar, G. and Tripathy, R. 2009. Influence of heat stress on genome of grass pea (Lathyrus sativus L.). J. Environ. Biol. 30: 403-408

[29] Li, B., Sun, Q., Yu, X., Xie, Y., Hong, J., Zhao, X., Sang, X., Shen and Hong, F. 2015 .Molecular mechanisms of silk gland damage caused by phoxim exposure and protection of phoxim-induced damage by cerium chloride in Bombyx mori. Environ. Toxic. 30 (9) : 1102–1111

[30] Li, G., Qian, H., Luo, X., Xu, P., Yang, J., Liu, M. and Xu, A. 2016. Transcriptomic Analysis of Resistant and Susceptible Bombyx mori Strains Following BmNPV Infection Provides Insights into the Antiviral Mechanisms. Int. J. Gen, : 1-10

[31] MacGown, M.W. and Sikorowski, P.P. 1982. Anatomy of the digestive system of Heliothis zea (Lepidoptera; Noctuidae) Larvae. Mississippi. Mafes. .

[32] Mahesha, H.B., Kasmaei, F.G. and Thejaswini, P.H. 2013. Studies on the correlation between DNA, RNA and proteins of Bombyx mori L . Inter. J. Biol. Pharm. All. Sci. 2(7): 1526-1534

[33] Mayurama, M. and Azuma, M. 2015. Aquarins are expressed in the columnar cells of the midgut epithelium of the silkworm, Bombyx mori. J insect. Biotech. Seric. 84:55-61

[34] Mitra, S. 2003. Sample Preparation Techniques in Analytical Chemistry. A John Wiley & Sons, Inc., Inc., Hoboken, New Jersey

[35] Mondal, M., Trivedy, K. and Kumar, S.N. 2007. The silk proteins, sericin and fibroin in silkworm, Bombyx mori Linn.Areview. Caspian J. Env. Sci. 5(2): 63-76

[36] Nagaraju, J., and Goldsmith, M.R. 2002. Silkworm genomics-progress and prospects, Curr. Sci. 83(4) : 415-425

[37] Nagasawa, H., Kataoka, H., Hori, Y., Isogai, A., Tamura, S., Suzuki, A., Guo, F., Zhong, X., Mizoguchi, A. Fujishita, M., Takahashi, S. Y., Ohnishi, E. and Ishizaki, H. 1984. Isolation and some characterization of the prothoracicotropic hormone from Bombyx mori. Gen. and Com. End. 53(1) : 143-152

[38] Nagasawa, H., Kataoka, H., Isogai, A., Tamura, S., Suzuki,A., Mizoguchit, A., Fujiwarat, Y., Suzuki, A., Takahashi, S.Y., and Ishizaki, H. 1986. Biochemistry Amino Acid Sequence Of A Prothoracicotropic Hormone Of The Silkworm Bombyx Mori (Insulin/Relaxin) Proc. Natl. Acad. Sci. Usa . 83 : 5840-5843

[39] Naguchi, A., Tokeshita, H. and Shigematsu, H. 1974. Interrelationship between the silk gland and other tissues in protein metabolism in the latest larval stages of the silkworm, Bombyx mori. J. Insect physiol. 20: 783-794.

[40] Noor, R.R. 1996. Genetika Ekologi. Bogor: Laboratorium Pemuliaan dan Genetika Ternak IPB

[41] Pal, A., Sharma, V.K. and Sisodia, N. 2014. Influence of Varying Temperature on Pupation from Larvae of Silkworm Bombyx Mori Syn. Char. Antimicr. Stu. Spectr. 3 (2) : 101-103

[42] Posnien, N,. Schinko, J.B., Kittelmann, S. and Bucher, G. 2010. Genetics, development and composition of the insect head e A beetle’s view. Arth. Str. Dev. 39 : 399-410.

[43] Prudhomme, J.C., Couble, P., Garel, J.P. and Daillie, J. 1985. Silk synthesis. In Comprehensive Insect Physiology, Biochemistry and Pharmacology; ed. G.A. Kurkut and L.I. Gilbert. Vol. 10. Pergamon Press, Oxford, New York, Toronto, Sydney, Paris, Frankfurt.

[44] Rahmathulla, V.K. 2012. Management of Climatic Factors for Successful Silkworm (Bombyx mori L.) Crop and Higher Silk Production: A Review. Hindawi Publishing Corporation Psyche pp. 1-12

[45] Rahmathulla, V.K., Mathur, V.B. and Geetha Devi, R.G. 2004. Growth and Dietary Efficiency of Mulberry Silkworm (Bombyx mori L.) Under Various Nutritional and Environmental Stress Conditions. Phi. J. Sci. 133 (1): 39-43

[46] Ramsay, J.A. 1976. The rectal complex in the larvae of lepidoptera. Philos. Trans. R. Soc. Lond. Biol. Sci. 274: 203-226.

[47] Regniere, R., Powell, J., Bentz, B., and Nealis, V. 2012. Effects of temperature on development, survival and reproduction of insects : Experimental design, data analysis and modeling. J. Insect Phy. 58 : 634-647

[48] Reinecke, J.P., Cook, B.J. and Adams, T.S. 1973. Larval hindgut of Manduca sexta (L.) (Lepidoptera: Sphingidae). Int. J. Ins. Mor. Emb. 2: 277-278.

[49] Sambrook, J. and Russel, D. 2001. Molecular Cloning: A Laboratory Manual, vol. 3, Cold Spring Harbor Laboratory Press, 3rd edition New York, NY, USA

[50] Sam-Eun, K. 1998. Silkworm Breeding. In Principle and Practice in Sericulture. National Sericulture and Entomology Research Institute. Rural Development Administration. Republic of Corea.

[51] Shimizu, M. 2000. Structural basis of silk fibre; in Structure of silk yarn. Vol., Biological and physical aspects. N. Hojo (ed.), Oxford & IBH Publication Co. Pvt. Ltd., New Delhi, pp.7-17.

[52] Smarason, S. V. and Smith, A. V. 2003. Method for desalting nucleic acids. United State patent US

[53] Snodgrass, R.E. 1935.Principles of insect morphology. McGraw-Hill Book Company, inc

[54] Srivastava, B.P. 1959. Morphology of the alimentary canal of the larva of Leucinodes orbonalis Guen. (Lepidoptera, Pyraustidae). Agr. Ins. 25: 188-200.

[55] Suzuki, Y. and Brown, D. D. 1972. Isolation and Identification of the messenger RNA for silk fibroin from Bombyx mori. J. Mol. Biol. 63: 409-429

[56] Suzuki, A., Nagasawa, H., Matsumoto, S., Kataoka, H., Isogai, A., Tamura, S., Ishizaki, H., Skurai, S., Fugo, H., Sonobe, H. and Ogura, N. 1983. Neurohormone in Silkworm, Bombyx mori. Annals International Geophysical Year. Natural Products with insect growth and behavior regulatory activity. 18 : 97-102

[57] Tsubota, T., Tomita, S., Uchino, K., Kimoto, M., Takiya, S., Kajiwara, H., Yamazaki, T. and Sezutsu, H. 2016. A Hox Gene, Antennapedia, Regulates Expression of Multiple Major Silk Protein Genes in the Silkworm Bombyx mori. J. Biol. Chem. 2016 291: 7087-.7097

[58] Vessaro-Silva. S.A., Brancalhao, R.M.C., Baggio, M.P.D. and Ribeiro, L.F.C. 2014. Cells of the rectum of Bombyx mori affected by experimental inoculation with Alphabaculovirus . Genet. Mol. Res. 13 (3): 6885-6891

[59] Weber, N.A. 1966. Fungus-Growing Ants. Science 153 (3736) : 587-604

[60] Wink, M. 2006. An Introduction to Molecular Biotechnology: Molecular Fundamentals, Methods and Application in Modern Biotechnology, Wiley-VCH, Weinheim, Germany

[61] Wu, S., Tong, X., Peng, C., Xiong, G., Lu, K., hu, H., Tan, D., Li. C., Han, M., Lu, C. and Dai, F. 2016. Comparative analysis of the integument transcriptomes of theblack dilute mutant and the wild-type silkworm Bombyx mori. Sci. Rep :1-11

[62] Wu, Y., Tingcai, C., Chun, L., Duolian, L. and Quan, Z. 2016. Systematic Identification and Characterization of Long Non-Coding RNAs in the Silkworm, Bombyx mori. PLoS One 11(1) : 1-10

[63] Yassin, H. M., Mostafa, S.S., Shokry, A.M., Salama, M.S. and Shahin, M.A. 2010. Identification, Isolation And Cloning Of cDNA Encoding Aminopeptidase From The Midgut Of The Egyetian Cotton Leaf Worm That Serves As A Receptor For Cry Toxin. Egypt. J. Gen. Cyt. 39 (2): 179-192

[64] Yu, X., Sun, Q., Li, B., Xie, Y., Zhao, Y., Hong, J., Sheng, L., Sang, X., Gui, S., Wang, L., Shen, W. and Hong, F. 2015. Mechanisms of larval midgut damage following exposure to phoxim and repair of phoxim-induced damage by cerium in Bombyx mori. Env. Tox. 30 (4) : 452-460