International Journal of Scientific & Technology Research

IJSTR@Facebook IJSTR@Twitter IJSTR@Linkedin
Home About Us Scope Editorial Board Blog/Latest News Contact Us

IJSTR >> Volume 5 - Issue 12, December 2016 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Microbiological And Antibiotic Resistance Pattern Analysis Of Wash Water From Tea Vendors Of Urban Areas In Bangladesh

[Full Text]



Safirun Pervin, Nuzhat Jerin, Tasmia Farzana, Shakila Sultana, Asif Hayat Zilani, S.M. Ariful Alam, Jahidul Islam, Fatema-E-Jannat, Suvamoy Datta



Antibiotic Resistance, Bacteriological analysis, Hygienic Condition, pathogens



Tea is the most widely consumed beverage in the world next to water. For selling food and drinks in Bangladesh a large number of people are involved. This study was performed to determine the microbiological quality of tea vendors in different area of Dhaka city. A total of 50 street tea vendors were randomly selected for this study. Samples were collected from Tea cup, vendor hand and water of each tea vendors. All samples were assessed for the presence of total bacterial count, total Enterobacteriaceae, total coliform count and total Staphylococcus aureus. The presence of Salmonella and Vibrio species were determined following appropriate enrichment and culture method. Cup and hand samples collected showed significantly higher count of total heterotrophic bacteria. Prevalence of Vibrio spp. and Salmonella spp., Staphylo-coccus aureus has been on higher side as compare to the Enterobacters and Klebsiella. Antibiotic sensitivity pattern of the S. aureus isolates shows that the highest sensitivity was observed against Streptomycin whereas high resistance observed against Amoxicillin. Contamination of tea vendor samples with pathogenic and resistant bacteria indicate their poor quality and unacceptability as drinks. This could be a threat to the consumer’s health and require immediate attention in order to control any outbreak of food and water borne diseases. Results of this concise study indicate that water testing is significant approach to ensure the supply and availability of contaminated-free water for tea preparation and processing.



[1] K. Chang, “World Tea Production and Trade: Current and Future Development. Proceedings and Abstracts of International Tea Symposium”,2014.

[2] Sivapalasingam and Sumathi, “Fresh produce: a growing cause of outbreaks of foodborne illness in the United States, 1973 through 1997.” Journal of Food Protection, vol. 67, no. 10, pp. 2342-2353, 2008.

[3] World Health Organization. Guidelines for drinking-water quality: recommendations. Vol. 1. World Health Organization, 2004.

[4] World Health Organization. Guidelines for Drinking-water Quality 2nd Edition, Volume 3: Surveillance and Control of Community Supplies, 1997.

[5] JMP, WHO UNICEF. Progress on Drinking Water and Sanitation: Special Focus on Sanitation, 2008.

[6] F.Tasnim, M. A. Hossain, K. M. Hossain, D. Lopa and K.M. Haque, "Quality assessment of industrially processed fruit juices available in dhaka city, bangladesh." Malaysian journal of nutrition, vol.16, no.3, pp. 431-8, 2010.

[7] Agard and Lavaughn, “Microbial quality of water supply to an urban community in Trinidad. Journal of Food Protection,”vol. 65, no. 8, pp. 1297-1303, 2002.

[8] A. Mankee, “Bacteriological quality of “doubles” sold by street vendors in Trinidad and the attitudes, knowledge and perceptions of the public about its consumption and health risk. Food microbiology”, vol. 20, no. 6, pp. 631-639, 2003.

[9] Buchanan, L. Robert and C. W. Richard, “Risk assessment: a means for linking HACCP plans and public health." Journal of Food Protection, vol.61, no.11, pp. 1531-1534, 1998.

[10] J. P. S. Cabral, “Water microbiology. Bacterial pathogens and water.” International Journal of Environmental Research and Public Health, vol. 7, no. 10, pp. 3657-3703, 2010.

[11] Armstrong, L. JOHN, “Antibiotic-resistant bacteria in drinking water.” Applied and Environmental Microbiology, vol. 42, no. 2, pp. 277-283, 1981.

[12] Sivapalasingam and Sumathi, “Fresh produce: a growing cause of outbreaks of foodborne illness in the United States, 1973 through 1997.” Journal of Food Protection, vol. 67, no. 10, pp. 2342-2353, 2004.

[13] R. T. Noble, D.F. Moore, M.K. Leecaster, D. Charles, D. C. McGee and B.S. Weisberg, “Comparison of total coliform, fecal coliform, and enterococcus bacterial indicator response for ocean recreational water quality testing,” (2003), Water research, vol. 37, no. 7, pp. 1637-1643, 2003.

[14] H.G. John, “Bergey's Manual of determinate bacteriology”, 1994.

[15] J. A.Washington, “Susceptibility tests: agar dilution”, Manual of clinical microbiology, vol. 4, pp. 967-971, 1985.

[16] A. W. Bauer, W. M. Kirby, J. C. Sherris and M. Turck, “Antibiotic susceptibility testing by a standardized single disk method”, American Journal of Clinical Pathology, vol. 45, no. 4, pp. 493–496, 1966.

[17] Black, G. Jacquelyn, Microbiology: principles and explorations. John Wiley & Sons, 2008.

[18] M. Ahmed, and S. Uddin, “Microbiological quality of local market vended freshly squeezed fruit juices in Dhaka city, Bangladesh”, Bangladesh Journal of Scientific and Industrial Research, vol. 44, no. 4, pp. 421-424, 2009.

[19] A.A. Othman, S. A. Musharaf, N.M. A. Daghri, S. Yakout, K.M. Alkharfy, Y.S. Saleh, O.S.A. Attas, M.S. Alokail, O. Moharram, S. Sabico, S. Kumar and G. P. Chrousos , “Tea and coffee consumption in relation to vitamin D and calcium levels in Saudi adolescents”, Nutrition journal, vol. 11, no. 1, pp. 1, 2012.

[20] Research report on – “Water Borne Diseases Caused by Using Surface water”. Habitat for Humanity International-Bangladesh, 2012.

[21] Parashar, D. Umesh, S. B. Joseph and I. G. Roger, “The global burden of diarrhoeal disease in children”, Bulletin of the World Health Organization, vol. 81, no. 4, pp. 236-236, 2003.