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IJSTR >> Volume 7 - Issue 12, December 2018 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Radio-Sensitivity Test Of Acute Gamma Irradiation Of Two Variety Of Chili Pepper Chili Bangi 3 And Chili Bangi 5

[Full Text]

 

AUTHOR(S)

Aisha, A.H, Rafii, M.Y, Rahim H.A, Juraimi A.S, Misran, A, Oladosu Yusuff

 

KEYWORDS

Chili; gamma ray; growth parameters; Lethal Dose; mutation breeding.

 

ABSTRACT

Induced mutation is a powerful method to generate heritable variation by exposing plant materials such as seeds, seedlings or other plant parts to physical or chemical mutagens. Mutagenesis remarkably enhanced the agronomical and physiological characters such as pest and diseases resistance, improve yield and yield related traits in crops. Gamma radiation is the most extensively use mutagen to induce mutation in plant breeding because of cost effectiveness, high mutation frequency, and reproducibility. Based on this background, this study was conducted on two chili varieties namely, Bangi3 and Bangi5 by exposing them to different dose of gamma ray 100, 200, 300, 400, 500, 600, 700, 800, 900 and 1000 Gy to determine LD50 and the morph-physiological responses. The irradiated seeds along with the control seeds were sown in nursery bed in the greenhouse in field 16 at Faculty of Agriculture, Universiti Putra Malaysia. Lethal dose LD50 of two chili varieties was determined. The effect of gamma- ray on shoot length, length of root, fresh and dry weight of shoot and root, survival plants were assayed. The result revealed that the low gamma irradiation doses enhanced some of the growth parameters, however high doses severely affect these traits. Therefore, mutagenic dose of 310 and 447 Gy were estimated as the LD50 for Bangi3 and Bangi5 variety, respectively.

 

REFERENCES

[1] Kharkwal, MC., & Shu, Q.Y. (2009). The role of induced mutations in world food security. Shu QY (eds) In: Induced plant mutations in the genomics era. Food and Agriculture Organization of the United Nations, Rome, pp. 3338.

[2] Gopalakrishnan, R., & Selvanarayanan, V. (2009). Preliminary evaluation of mutant tomato accessions for resistance against whitefly Bemisia tabaci gennadius (Aleyrodidae: Hemiptera). Advances in Biological Research, 3(5-6), 159161.

[3] Tomlekova, N.B., Timina, O.O., Timin, O.Y. (2009). Achievement and perspectives of sweet pepper breeding towards high Beta Carotene. Acta Horticulturae, 1, 205209.

[4] Oladosu Y, Rafii MY, Abdullah N, Malek MA, Rahim HA, Hussin G, Kareem I, 2015. Genetic variability and diversity of mutant rice revealed by quantitative traits and molecular markers. Agrociencia. 49:249−266.

[5] Nascimento, KS., Rego, MM., Nascimento, AM. & Rego, AR. (2015). Ethyl methane sulphonate in the generation of genetic variability in Capsicum. Acta Horticulturae, 1087: 249-254

[6] Oladosu, Y., Rafii, M. Y., Abdullah, N., Hussin, G., Ramli, A., Rahim, H. A., & Usman, M. (2016). Principle and application of plant mutagenesis in crop improvement: a review. Biotechnology & Biotechnological Equipment, 30(1), 116.

[7] Paran, I., Borovsky, Y., Nahon, S., & Cohen, O. (2007). The use of induced mutations to study shoot architecture in Capsicum. Israel Journal of Plant Sciences, 55 (2), 125131.

[8] Omar, SR., Ahmed, OH., Saamin, S., & Majid, NMA. (2008). Gamma radiosensitivity study on chili (Capsicum annuum). American Journal of Applied Science, 5(2), 6770.

[9] Wi SG., Chung BY., Kim JS., Kim JH., Baek MH., Lee JW. & Kim YS. (2006). Effects of gamma irradiation on morphological changes and biological responses in plants. Micron, 38: 553-564.

[10] Ulukapi, K., Ozdemir, B., & Onus, A. N. (2015). Determination of Proper Gamma Radiation Dose in Mutation Breeding in Eggplant (Solanum melongena L.). Advances in Environmental and Agricultural Science, pp. 149153.

[11] Tabasum, A., Cheema, AA., Hameed, A., Rashid, M., & Ashraf, M. (2011). Radio sensitivity of rice genotypes to gamma radiations based on seedling traits and physiological indices. Pakistan Journal of Botany, 43(2), 12111222.

[12] Oladosu Y, Rafii MY, Abdullah N, Abdul Malek M, Rahim HA, Hussin G, Kareem I, 2014. Genetic variability and selection criteria in rice mutant lines as revealed by quantitative traits. Scientific World J. http://dx.doi.org/10.1155/2014/190531

[13] Gaswanto, R., Syukur, M., Purwoko, BS., & Hidayat, SH. (2016). Induced mutation by gamma rays irradiation to increase chilli resistance to begomovirus. Agrivita, 38(1), 24.

[14] Kon, E., Ahmed, OH., Saamin, S., & Majid, NM. (2007). Gamma radiosensitivity study on long bean (Vigna sesquipedalis). American Journal of Applied Science, 4(12), 10901093.