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IJSTR >> Volume 2- Issue 1, January 2013 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Starvation And Associated Neuropathies In The Hippocampal Formation Of Adult Wistar Rats (Rattus Norvegicus)

[Full Text]



Fakunle P. B, Ajibade A. J



KEYWORDS:- Hippocampus, Starvation, Rats, Pyramidal Neurons, Memory



ABSTRACT:- Neurohistological integrity of hippocampus when exposed to starvation was investigated in thirty adult wistar rats of average weight 200g . They were acclimatized for 2weeks on standard laboratory mouse chow and provided water ad libitum. They were subsequently randomly distributed into 3 groups (n=10) of Control C, Treatment T1 and Treatment T2. Treatments T1 and T2 animals were exposed to starvation periods of 3 and 14days respectively and sacrificed by whole-body intracardiac perfusion fixation under gravity for routine histological techniques. Statistical analysis showed significant body weight loss P0.05 in T1(Mean±sem, 194.8±6.1)g compared to the control section with Mean±sem,225.0±6.21g.Histoarchitecture of treatment group T2 revealed distinct hippocampal subfields of CA1,CA2,CA3 and the DG (Dentate Gyrus) with pyknotic pyramidal cells and scantily distributed glial cells compared to the control, these findings were less marked in the T1 cells while the control group cells appeared normal. Also, significantly(P<0.05) reduced neuronal population of 11%, 8% and 14 % in CA1, CA2 and CA3 subfields respectively for Treatment group T1 compared to the control group.The neuronal transverse diameter for the pyramidal cells revealed statistically significant(P<0.05) values Mean±SEM (1.20±0.01)µm was obtained in treatment group T1 compared to the control group.Long term starvation has severe and deleterious effects on the cellular integrity of hippocampus, it may thus underline problems associated with memory and learning.



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