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Determination Of The Curcumin Pigment In Extract Curcuma Domestica Val From South Sulawesi, Indonesia, By High Performance Liquid Chromatography
[Full Text]
AUTHOR(S)
Sri Hastati, Veni Hadju, Gemini Alam, Nusratuddin
KEYWORDS
Index Terms: curcuma domestica val., turmeric, curcumin, hplc,
ABSTRACT
Abstract: Indonesia is a country endowed with a variety of medicinal plants with strong potential for therapeutic application, curcuma domestica val is called kunyit is one of the most popular medicinal herbs.Its important active ingredient responsible for the biological activityofcurcuma, through the major activity is antyinflammatory. Curcuma has also been reported to possess antioxidant, antiallergic,wound healing,antibacterial,antifungal and anti tumor activity.The yellow colour of curcuma is mainly due to the presence of polyphenolic curcuminoids. This experiment was aimed to determine analysis compound of curcuminoid form analysis using HPLC,LC-10AT,Shimadzu,column C-18 VP-ODS, acetonitril:acetic acid:aquabides as mobile phase,flow rate 1 ml/min and detection at 425nm.Thus the analytical method using HPLC for curcumin were feasible for quantitative analysis. The curcuminoid content from Extract Curcuma domestica Val had the highest curcumin (16.1%) followed metoxycurcumin (3.2%) and bisdemetoxycurcumin 2.8%
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