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IJSTR >> Volume 5 - Issue 5, May 2016 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Synthesis Of Three Analogues Of Trypsin-Modulating Oostatic Factor (TMOF) And Screening Of Their Insecticidal Properties Towards Cabbage Cluster Caterpillar

[Full Text]

 

AUTHOR(S)

Rani Maharani, Ari Hardianto, Safri Ishmayana, Eka Fitri Yanti, Devia Irma Melati, Daniel Sihotang, Lindung Tri Puspasari, Danar Dono

 

KEYWORDS

Trypsin-modulating oostatic factor, TMOF, Aedes aegypty, Crocidolomia pavonana, solid-phase peptide synthesis, 2-chlorotrityl chloride resin, DIC/Oxyma.

 

ABSTRACT

Three analogues of TMOF, [PP (H-Pro-Pro-OH), PPPPPP (H-Pro-Pro-Pro-Pro-Pro-Pro-OH), and APPPPPP (H-Pro-Pro-Pro-Pro-Pro-Pro-OH)], were successfully synthesised and screened for their insecticidal properties against cabbage cluster caterpillar (Crocidolomia pavonana). All compounds were prepared through Fmoc-based solid-phase peptide synthesis (SPPS) method. The synthesis employed chlorotrityl resin as solid support. A combination of N,N-diisopropylcarbodiimide (DIC) and ethyl 2-cyano-2-(hydroxyimino)acetate (oxyma) was chosen as coupling reagent in the synthesis. The peptide was cleaved by using TFA cocktail. All peptide crudes were purified by using reversed-phase flash chromatography with octadecyl silane (ODS) as stationary phase. All purified peptides were characterized by using TOF-ESMS and their purity was analysed using thin layer chromatography and analytical RP-HPLC. The synthesised TMOF analogues, together with TMOF, were biologically tested towards the (C. pavonana) at 1000 ppm. The results indicated that TMOF and the analogues were less effective for the cabbage cluster caterpillar, compared to previously reported activity of TMOF towards Aedes aegypty.

 

REFERENCES

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