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IJSTR >> Volume 9 - Issue 6, June 2020 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Optimization And Cost-Effective Production Of Fungal Glucose Oxidase Using Palm Jaggery

[Full Text]

 

AUTHOR(S)

Uppalapati pavankalyan, Annavarapu Yasaswi, Atchukola Teja, Giridhar Kanuri, Chandrasekhar Chanda

 

KEYWORDS

Aspergillus niger. Benzoquinone assay, Column chromatography, CM Sephadex, Glucose oxidase, Media optimization, Palm Jaggery

 

ABSTRACT

Glucose oxidase (GOx) belongs to oxido-reductase class of enzyme produced by few fungal strains and insects. Glucose oxidase has several applications in food, pharmaceutical and biotechnological industries. Glucose oxidase is widely used in diagnostics and biosensors in determining glucose levels. Current study focussed on cost effective production, purification and characterization of glucose oxidase isolated from fungal strain Aspergillus niger grown on palm jaggery. Extracellular fungal proteins were fractionated using cation exchange column chromatography and purified glucose oxidase activity was tested with modified benzoquinone activity assay. Approximately 560 IU/ml of glucose oxidase activity was observed using 1% palm jaggery as primary carbon source in submerged fermentation.

 

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