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IJSTR >> Volume 9 - Issue 6, June 2020 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Structural Elucidation And Analysis Of Toxic Pyrrolizidine Alkaloids From Staple Foods Of North West Tigray, Northern Ethiopia

[Full Text]

 

AUTHOR(S)

Tsegay Hiwot, Abrha Birhan

 

KEYWORDS

pyrrolizidine alkaloids; veno-occlusive disease, Ehrlich’s reagent, Teff, Enjera Tsaeda-emba, Adishukor, Mekayho, thin layer chromatograpy and GC-MS

 

ABSTRACT

The objective of this study was to identify the hepatotoxic pyrrolizidine alkaloids (PAs) in grains processed food and animal products in three villages of the North West zone of regional state of Tigray, northern Ethiopia. By chemical test using Ehrlich’s reagent, and separation by thin layer chromatograpy (TLC) and gas chromatography-mass spectrometry (GC-MS) spectrometric analysis, three toxic PAs, echinatine, clivorine, and europine, were identified in the Teff and Enjera food samples taken from three veno-occlusive disease affected villages of Tsaeda-emba, Adishukor and Mekayho in northern Ethiopia. PAs are phytochemicals present in more than 6000 plant species worldwide; approximately half of the PAs are hepatotoxic, genotoxic, and carcinogenic. PAs induce acute hepatotoxicity with the typical symptom known as hepatic sinusoidal obstruction syndrome (HSOS). To date, three representative carcinogenic PAs, monocrotaline, riddelliine, and lasiocarpine, have been classified as possible human carcinogens (Group 2B) by the International Agency for Research on Cancer (IARC). Therefore, it is important to determine the sources of exposure and study the mechanism of action. As such, the identification of three toxic PAs in the villages of Ethiopia with people suffering from HSOS is highly important.

 

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