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IJSTR >> Volume 9 - Issue 6, June 2020 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Optimization Of Process Parameters for Synthesis of Silver Nanoparticles Using Leaf Extract of Tridax Procumbent and Its Biotechnological Applications

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Laxmikant R. Patil*, Anil R. Shet, Arati G. Lohar, Gururaj B.Tennalli, Sharanappa A., V. S. Hombalimath, Umesh Deshannavar



Tridax procumbens, Phytochemical screening, Silver nanoparticles, UV-spectroscopy, Antimicrobial activity, Antioxidant activity, H2O2 detection.



Green synthesis of nanoparticles have acquired a lot of importance because of their cost effectiveness and environmental friendly nature. In this work, optimization of process parameters for the synthesis of silver nanoparticles (AgNPs) using leaf extracts of Tridax procumbens was done. Ultraviolet-Visible (UV-Vis) spectrophotometer was used for the confirmation of AgNP synthesis. The effect of process parameters like quantity of leaf extract, silver nitrate (AgNO3) concentration, incubation time, temperature and pH were studied. The optimum conditions were found to be 1ml of leaf extract, 10mM concentration of silver nitrate, pH of 9, incubation time of 180minutes and temperature of 40oC. The synthesized AgNPs were examined for antimicrobial property against two Gram-positive bacteria and two Gram-negative bacteria. Highest antibacterial activity was noticed for Gram negative bacterium Pseudomonas aeruginosa. Antioxidant properties were analyzed by DPPH and H2O2 assay and were found to be significant for the synthesized AgNPs. Further AgNPs were used for the qualitative detection of Hydrogen peroxide.



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