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IJSTR >> Volume 9 - Issue 6, June 2020 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Fungal Diversity In Leaves And Stems Of Neem (Azadirachta Indica)

[Full Text]



Nasiya R. Al-Daghari, Sajeewa S.N. Maharachchikumbura, Dua’a Al-Moqbali, Nadiya Al-Saady, and Abdullah Mohammed Al-Sadi



Endophytic fungi, fungal diversity, Medicinal plants, endophytes



A study was conducted to examine fungal endophytes present in leaf and stem tissues of Azadirachta indica. A total of 65 fungal isolates were recovered from 144 neem plant segments (leaf and stem tissues) showing no disease symptoms or physical damage. The samples were collected from 8 different locations in Oman during the year 2017. The isolates were classified into 15 different morphotypes according to culture characteristics and were identified based on rDNA ITS sequence analysis. In total, 23 taxa belonging to 15 genera were identified, all belonging to the ascomycetes classes Dothideomycetes, Sordariomycetes and Eurotiomycetes. Class Dothideomycetes was dominant and was represented by six families: Cladosporiaceae, Saccotheciaceae, Botryosphaeriaceae, Didymellaceae and Pleosporaceae, followed by Sordariomycetes (Chaetomiaceae, Microascaceae and Nectriaceae) and Eurotiomycetes (Trichocomaceae and Aspergillaceae). The most frequently isolated taxa were Cladosporium sphaerospermum, Alternaria spp and Aspergillus niger. Leaf samples yielded more fugal taxa compared to stem, and our data show that neem contains taxonomically diverse fungal endophytes. Furthermore, this is the first report of Aspergillus caespitosus, Curvularia geniculate, Curvularia subpapendorfii, Leptosphaerulina australis and Microascus cinereus from Oman.



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