IJSTR

International Journal of Scientific & Technology Research

Home About Us Scope Editorial Board Blog/Latest News Contact Us
0.2
2019CiteScore
 
10th percentile
Powered by  Scopus
Scopus coverage:
Nov 2018 to May 2020

CALL FOR PAPERS
AUTHORS
DOWNLOADS
CONTACT

IJSTR >> Volume 1 - Issue 7, August 2012 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



An In Vitro Study of The Antifungal Activity of Silver/Chitosan Nanoformulations Against Important Seed Borne Pathogens

[Full Text]

 

AUTHOR(S)

Pawan Kaur, Rajesh Thakur# and Ashok Choudhary

 

KEYWORDS

Keywords: Antifungal, Chitosan, mycelium, nanoformulations, phytopathogens, seed borne, silver.

 

ABSTRACT

Abstract- This research is reports the fungicidal properties of nano-size silver/chitosan nanoformulations (NFs) used as an agent for antifungal treatment of various seed borne plant pathogens. Fungal phytopathogens, especially seed borne disease causing species, Rhizoctonia solani, Aspergillus flavus, Alternaria alterneta were isolated form chickpea seeds. Differences were observed in the antifungal activity of the silver nanoparticle, chitosan nanoparticles and silver /chitosan nanocomposite, upon the mycelial growth and zone of inhibition of the fungi. Extent of fungal inhibition was derived using the above information, in order to evaluate the antifungal efficacy of these nanoformulations against pathogens. Tests for the fungal growth revealed that the NFs showed significant inhibition effectiveness. This study suggests the possibility to use silver/chitosan NFs as an alternative to fungicides for controlling seed borne phytopathogens.

 

REFERENCES

[1] M.S. Homedes, V., Sanchis, V., Ramos, A.J. and Magan, N. “Impact of Fusarium moniliforme and F. proliferatum colonisation of maize on calorific losses and fumonisin production under different environmental conditions”. Journal of Stored Product Research, (1999), vol. 35, pp. 15 – 26.

[2] G.R. Janardhana, K.A. Raveesha, and H.S. Shetty, “Mycotoxin contamination of maize grains grown in Karnataka (india)”. Food Chemical Toxicology, (1999), vol. 37, pp 863 – 868.

[3] R. chandra, and A.K. sarbhoy, “Production of aflatoxins and zearalenone by the toxigenic fungal isolates obtained from stored food grains of commercial crops”. Indian Phytopathology , (1997) ,vol. 50, pp. 458-68 .

[4] F. Galvano, A. Piva, A. Ritieni, and G. Galvano, “Dietary strategies to counteract the effect of mycotoxins: A review”. Journal of Food Protection, (2001), vol. 64, pp. 120 – 131.

[5] K. Datta, R. Velazhahan, N. Oliva, I. Ona, T. Mew, G.S. Khush, S. Muthukrishnan, and S.K. Datta, “Over-expression of the cloned rice thaumatin-like protein (pr-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease”. Theor. Appl. Genet., (1999), vol. 98, pp. 1138-1145.

[6] H.R. Dillard, and R.G. Grogan, “Relationship between sclerotial spatial pattern and density of Sclerotinia minor and the incidence of lettuce crop”. Phytopathology, (1985), vol. 75, pp. 90-94.

[7] J.R. Coley-Smith, “Survival of plant pathogenic fungi in soil in the absence of host plants. Soil-borne Plant Pathogens, ed. by Schippers, B., and Gams, W. Academic Press, London pp. 39-57, 1979.

[8] S. Nishimura, “Host specific toxins from Alternaria alternata, problems and prospects”. Proc. Jpn. Acad, (1980), vol 56 (b), pp. 362-366.

[9] C.A. Harris, M.J. Renfrew, and M.W. Woolridge, “Assessing the risk of pesticide residues to consumers: recent and future developments”. Food Additives and Contamination, (2001), vol. 18, pp. 1124-1129.

[10] Y.K. Jo, B.H. Kim, and G. Jung, “Antifungal activity of silver ions and nanoparticles on phytopathogenic fungi”. Plant Dis., (2009), vol. 93, pp.1037-43.

[11] H.J. Park, S.H. Kim, H.J. Kim, and S.H. Choi, “A new composition of nanosized silica-silver for control of various plant diseases”. Plant Pathol. J, (2006), vol. 22, pp.295-302.

[12] J.S. Min, K.S. Kim, S.W. Kim, J.H. Jung, K. Lamsal, S.B. Kim, M. Jung, and Y.S. Le, “Effects of colloidal silver nanoparticles on sclerotium-forming phytopathogenic fungi”. Plant Pathol. J, (2009), vol.25, pp.376-80.

[13] E.J. Arul, A. Asselin, and N. Benhamou, “Antifungal activity of chitosan on postharvest pathogens induction of morphological and cytological alterations in Rhizopus stolonifer”. Mycol. Res., (1992), vol. 96, pp. 769–779.

[14] Y. Baba, T. Yamashita, Y. Kawano, and Y. Uchida, “Antifungal activity of aqueous soluble chitosan derivatives on Fusarium and Verticillium”. Nippon Kagaku Kaishi, (1996), vol.1, pp. 48–53.

[15] M.V.B. Reddy, J. Arul, E. Ait-Barka, P. Angers, C. Richard, and F. Castaigne, “Effect of chitosan on growth and toxin production by Alternaria alternata f. sp. lycopersici”. Biocontrol Sci. Technol., (1998), vol. 8, pp. 33–43.

[16] E.I. Rabea, M.E.T. Badawy, C.V. Stevens, G. Smagghe, and W. Steurbaut, “Chitosan as antimicrobial agent: applications and mode of action”. Biomacromolecules, (2003), vol. 4, pp.1457–1465.

[17] S. Sautista-Banos, A.N. Hernandez-Lauzardo, M.G. Velazquez-Del Valle, M. Hernandez-Lopez, E. Barka, E. Bosquez-Molina, and C.L. Wilson, “Chitosan as a potential natural compound to control pre and postharvest diseases of horticultural commodities”. Crop Prot., (2006), vol.25, pp. 108–118.

[18] C.R. Allan, and L.A. Hadwiger, “The fungicidal effect of chitosan on fungi of varying cell wall composition”. Exp. Myco., (1979), vol. 3, pp. 285–287.

[19] A. Zakrzewska, A. Boorsma, S. Brul, K.J. Hellingwerf, and F.M. Klis, “Transcriptional response of Saccharomyces cerevisiae to the plasma membrane-perturbing compound chitosan”. Eukaryot. Cell., (2005), vol. 4, pp. 703–715.

[20] P. Kaur, R. Thakur and A. Chaudhury, “Synthesis of chitosan-silver nanocomposites and their antibacterial activity. Proceed. ICNANO , D.U, New Delhi, India, pp. 548, 2011.

[21] Wen-Li Du, S.S. Niu, Y. L. Xu, Z. R. Xu, and Cheng-Li Fan, “Antibacterial activity of chitosan tripolyphosphate nanoparticles loaded with various metal ions”. Carbohydrate Polymers, (2009), vol. 75, pp. 385.

[22] V. Bobbarala1, P. K. Katikala, K.. C. Naidu and S. Penumaj, “Antifungal activity of selected plant extracts against phytopathogenic fungi Aspergillus niger f2723”. Indian Journal of Science and Technology, (2009) ,vol. 2, pp. 87-90.

[23] J. Singh, and N.N. Tripathi, “Inhibition of storage fungi of blackgram (vigna mungo) by some essential oils”. Flavour and Fragrance J,(1999), vol. 14, pp. 1-4.