IJSTR >> Volume 7 - Issue 10, October 2018 Edition

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AUTHOR(S)

In Rahmi Fatria Fajar, Swasono R Tamat, Deni Rahmat

KEYWORDS

collagen, solid lipid nanoparticle (SLN), scales and skin of red snapper, penetration test

ABSTRACT

until now, needs of collagen are fulfilled mostly from beef or poultry. As there are more diseases found in poultry, the source of collagen from the skin and scales of fish becomes one promising alternative. The observed collagen parameters include proximate analysis, SDS-Page and heavy metals. To strengthen penetration of collagen, the application is made in form of solid lipid nanoparticles (SLN). SLN is made from Brij (5, 6, 7%) as the surfactant and Glycerol monostearate (GMS) (4, 5, 6%) as the cosurfactants and high-speed homogenization to produce a high penetration power. In-vitro penetration test is conducted by using Franz diffusion cells and mouse skin membrane. SDS-Page analysis shows various types of peptides contained in collagen with molecular weight of 100, 80, 70, 60, and 50 kDa. SLN compounds also meet the requirements which includes spherical morphology, particle size of 61.1 - 965.9 and zeta potential of (-3.16) – (12.37). The study concludes that collagen which is derived from the scales and skin of Red Snapper (Lutjanus malabaricus) can be applied in solid lipid nanoparticle (SLN) compound by using Brij (6%) and Glycerol monostearate (4%) as the surfactants and the cosurfactants, and having the highest penetration value of 897,125 µg/cm2.

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