mir-1792 Expression In Various Stages Of Chronic Hepatitis C Viral (HCV) Infection: Correlation With PEG-10 And PTEN

Background: Circulating microRNAs (miRNAs) have been reported as a promising epigenetic biomarker. miRNAs have significant diagnostic potential for hepatocellular carcinoma (HCC). In this study, expression of miR-17-92 in Hepatitis (C)-related Liver Cirrhosis and HCC was evaluated. miR-17-92 expression was assessed using quantitative real-time Polymerase chain reaction (RT-PCR) in 145plasma samples [45 healthy controls, 25 HCC patients, and 75 cirrhotic patients with various Child-Pugh stages]. The relation between miR-17-92 and paternally expressed 10 (PEG10) and Phosphatase and tensin homolog (PTEN) proteins were investigated. Results: Our study found a significant reduction in miR-17-92 expression (P<0.001) in HCV-infected patients [either cirrhotic or HCC patients] when compared with the healthy control group. miR-17-92 expression levels with a cutoff value of 1.175-fold change had a 64% sensitivity and 34.9% specificity. With a cutoff value of 8.5 ng/ ml, alpha-fetoprotein (AFP) had a high sensitivity (87.5%) and specificity (100%) in the detection of HCC. BothPEG10 (P<0.001) and PTEN (P<0.01) were significantly decreased in HCC patients as compared with the healthy controls. The detection of miR-17-92, PEG10, and PTEN is valuable in elucidating the complex regulatory mechanisms governing HCC tumorigenesis. Conclusions: The combined detection of these factors may have clinical importance in prognostic, diagnosis, judgment, and therapeutic options for primary HCC.A future prospective large-scale study is recommended to support our findings and prove the mechanistic connections between miR-17-92 and PEG10/PTEN levels and HCV disease manifestations.

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