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Development Of A Pig Skin Model To Test Nanocomposite Materials For Antibacterial Properties
[Full Text]
AUTHOR(S)
Katrina Rushford, Corey Gemelli, Gerri Wilson, Mohamed Asfour, Hamed Benghuzzi, Drew Hildebrandt, Joseph Lichtenhan, Michelle Tucci
KEYWORDS
ABSTRACT
INTRODUCTION: Polyhedral oligomeric silsesquioxane is making advancements towards medical application as an effective material for drug delivery. POSS is a unique molecule because of its structure. It is a class of organosilicon- three-dimensional compounds with cage frameworks and different degrees of symmetry. OBJECTIVE: Our objective was to introduce a biomedical application for POSS in area of drug delivery. For large open wounds it should have a capability to reduce bleeding and as well as provide the release of an antibiotics to reach minimal inhibitory concentrations at the site for infection coverage. If the treatment is successful, POSS can be applied in the field as a protective dressing that is capable of enhancing clot formation, prevent infection, and ultimately enhance tissue repair. METHODS: Two formulations of POSS were prepared and tested in vitro to release inhibitory concentration of Clindamycin. Clindamycin release was determined spectrophotometrically, and the MIC was determined using a bacterial pig skin wound model were determined. RESULTS: Minimal inhibitory concentration of Clindamycin was detected from both POSS formulation for up to 96 hours. In the pig skin wound model the POSS formulation without kaolin reduced the bacterial load more efficiently than POSS containing kaolin and was comparable to a triple antibiotic cream coverage. CONCLUSION: Nanocomposites of POSS can be formulated to limit bacterial growth and further reduction of bacterial load can be accomplished by attaching an antibiotic to the functional groups of the caged nanostructure of POSS.
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