Radio-Sensitivity Test Of Acute Gamma Irradiation Of Two Variety Of Chili Pepper Chili Bangi 3 And Chili Bangi 5

Induced mutation is a powerful method to generate heritable variation by exposing plant materials such as seeds, seedlings or other plant parts to physical or chemical mutagens. Mutagenesis remarkably enhanced the agronomical and physiological characters such as pest and diseases resistance, improve yield and yield related traits in crops. Gamma radiation is the most extensively use mutagen to induce mutation in plant breeding because of cost effectiveness, high mutation frequency, and reproducibility. Based on this background, this study was conducted on two chili varieties namely, Bangi3 and Bangi5 by exposing them to different dose of gamma ray 100, 200, 300, 400, 500, 600, 700, 800, 900 and 1000 Gy to determine LD50 and the morph-physiological responses. The irradiated seeds along with the control seeds were sown in nursery bed in the greenhouse in field 16 at Faculty of Agriculture, Universiti Putra Malaysia. Lethal dose LD50 of two chili varieties was determined. The effect of gamma- ray on shoot length, length of root, fresh and dry weight of shoot and root, survival plants were assayed. The result revealed that the low gamma irradiation doses enhanced some of the growth parameters, however high doses severely affect these traits. Therefore, mutagenic dose of 310 and 447 Gy were estimated as the LD50 for Bangi3 and Bangi5 variety, respectively.

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