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Inducing Somatic Embryogenesis Of Salak Sidempuan (Salacca Sumatrana Becc.) With The Addition Of Lysine
[Full Text]
AUTHOR(S)
Elimasni , Haryati, Khairiyah Khairuddin
KEYWORDS
Tissue culture, Lysine, Salacca sumatrana Becc., Somatic embryogenesis.
ABSTRACT
The research of inducing somatic embryogenesis from salak sidempuan (Salacca sumatrana Becc.) with adding of lysine had been conducted. This study aimed to determine the effect of lysine treatments on the growth and the development of somatic embryos of salak sidempuan (Salacca sumatrana Becc.). The samples collected directly at salak farmers at Padang Sidempuan, North Sumatra. MS Cultures Media were used complete with adding of amino acid lysine. The concentration of lysine were range from 0 mg/L; 10 mg/L; 20 mg/L; 30 mg/L; 40 mg/L and 50 mg/L. The results obtained were analyzed statistically by ANOVA analysis random design. The results showed the best survival rate found on 10 mg/L of lysine concentration., the best wet weight found on 40 mg/L of lysine concentration. This lysine concentration also had good ability to form somatic embryo of salak sidempuan. The histological analysis showed that somatic embryos formed at globular and heart–shape phase.
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