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IJSTR >> Volume 7 - Issue 6, June 2018 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Inducing Somatic Embryogenesis Of Salak Sidempuan (Salacca Sumatrana Becc.) With The Addition Of Lysine

[Full Text]

 

AUTHOR(S)

Elimasni , Haryati, Khairiyah Khairuddin

 

KEYWORDS

Tissue culture, Lysine, Salacca sumatrana Becc., Somatic embryogenesis.

 

ABSTRACT

The research of inducing somatic embryogenesis from salak sidempuan (Salacca sumatrana Becc.) with adding of lysine had been conducted. This study aimed to determine the effect of lysine treatments on the growth and the development of somatic embryos of salak sidempuan (Salacca sumatrana Becc.). The samples collected directly at salak farmers at Padang Sidempuan, North Sumatra. MS Cultures Media were used complete with adding of amino acid lysine. The concentration of lysine were range from 0 mg/L; 10 mg/L; 20 mg/L; 30 mg/L; 40 mg/L and 50 mg/L. The results obtained were analyzed statistically by ANOVA analysis random design. The results showed the best survival rate found on 10 mg/L of lysine concentration., the best wet weight found on 40 mg/L of lysine concentration. This lysine concentration also had good ability to form somatic embryo of salak sidempuan. The histological analysis showed that somatic embryos formed at globular and heart–shape phase.

 

REFERENCES

[1] Ashari, S. 2005. Meningkatkan Keunggulan Bebuahan Tropis Indonesia. Edisi Pertama. Andi. Yogyakarta.

[2] Blanc, G.N., Michaux, F., Teisson, C., Larder, L and Carron M.P. 1999. Effects of Carbohydrate Addition on the Induction of Embryogenesis in Havea brasiliensis. Plant Cell Tissue and Organ Culture. 59: 103-110.

[3] Ghimre, KB. Yu CY. Chung I. 2011. Direct shoot organogenesis and assessment of genetic stability in regenerants of Solanum aculeatissimum Jacq. Plant Cell Tissue Org Cult (Published on line 4 October 2011)

[4] Griga, M. 2002. Morphology and anatomy of Pisum sativum somatic embryos. Biol Plan. 45:173-192

[5] Gunawan, L.W. 1988. Teknik Kultur Jaringan. IPB. Bogor.

[6] Gunawan, L.W. 1995. Teknik Kultur In Vitro dalam Holtikultura. IPB. Bogor.

[7] Hadfi K, Speth V, Neuhaus G. 1998. Auxin-induced developmental patterns in Brassica juncea embryos. Development. 125:879-887

[8] Hadi, H., Indrianto, A dan Admojo, L. 2014. Perkembangan Penelitian Induksi Kalus Embriogenik pada Jaringan Vegetatif Tanaman Karet Klonal (Hevea brasiliensis Muell. Arg). Warta Perkaretan. 33(1): 20-26.

[9] Herwinaldo, D.C. 2010. Pengaruh Variasi Konsentrasi Sukrosa terhadap Pertumbuhan dan Induksi Embriogenesis Somatik Kultur Kalus Tapak Dara (Catharanthus roseus G. Don). [Skripsi]. Surakarta. Universitas Sebelas Maret, Program Sarjana.

[10] Husain, I. 2012. Induksi Protocorm pada Eksplan Bawang Putih pada Media MS minim Hara Makro dan Mikro yang ditambahkan Air Kelapa. JATT. 1(1): 28.

[11] Hussain SS. Rao AQ, Husnain T, Riazuddin S. 2009. Cotton somatic embryo morphology effect its conversion into plants. Biol Plant. 53: 307-311.

[12] Jimenez, V.M. 2001. Regulation of In Vitro Somatic Embryogenesis with Emphasis on the Role of Endogenous Hormones. R. Bras Physiol Veg. 13(2): 196-223.

[13] Jimenez, V.M. 2005. Involvement Of Plant Hormones And Plant Growth Regulators on in Vitro Somatic Embryogenesis. Plant Growth Regulation. 47: 110.

[14] Kasli. 2009. Upaya Perbanyakan Tanaman Krisan (Chrysanthemum sp.) secara in vitro. Jerami. 2(3): 122-124.

[15] Lazzeri PA, Hildebrand DF, Collins GB. 1987. Soybean somatic embryogenesis: effect of hormones and culture manipulations. Plant Cell Tissue and Org Culture. 10: 197-208.

[16] Meilvana, N.T. 2014. Analisis Histologi Embriogenesis Somatik dari Apikal Bud Kelapa Sawit (Elaeis guineensis Jacq.) var. Tenera. [Tesis]. Medan: Universitas Sumatera Utara, Program Pascasarjana.

[17] Parrot WA. 1991. Auxin stimulated somatic embryogenesis for immature cotyledons of white clover. Plant Cell Rep. 10: 17-21

[18] Purnamaningsih. 2002. Regenerasi Tanaman melalui Embriogenesis Somatik dan Beberapa Gen yang Mengendalikannya. Buletin Agro Bio. 5(2): 51-58.

[19] Purwito, A., Husni, A dan Rivai, R.R. 2014. Induksi Kalus dan Embrio Somatik Tanaman Jambu Biji Merah (Psidium guajava L.). Bul. Agrohorti. 2(1): 50.

[20] Rodriguez APM, Wetzstein HY. 1998. A morphological comparison of the initiation and development of pecan (Carya illinoiensis) somatic embryogenic cultures induced with naphtaleneacetic acid or 2,4-dichlorophenoxyacetic acid. Protoplasma. 204: 71-83

[21] Ruangsak, J and Dheeranupattana, S. 2014. Effects of L-Ornithine and L-Lysine on Alkaloid Production from in vitro Stemona sp. Chiang Mai J. Sci. 41(2): 334.

[22] Shoemaker RC, Amberger LA, Palmer GR, Oglesby L, Ranch JP. 1991. Effect of 2,4-dichlorophenoxyacetic acid concentration on somatic embryogenesis and heritable variation in soybean (Glycine max (L.) Merr.). In Vitro Cell Dev Biol-Plant. 27:84-88

[23] Soetomo, A.H.M. 2001. Teknik Bertanam Salak. Sinar Baru Algesido. Bandung.

[24] Sofiari E, Raemaker CJJM, Kanju E, Danso K, van Lammeren AM, Jacobsen E, Visser RGF. 1997. Comparison of NAA and 2,4-D induced somatic embryogenesis in Cassava. Plant Cell Tissue Org Culture. 50: 45-56

[25] Sumaryono., Riyadi I., Kasi P.D., Ginting G. 2007. Pertumbuhan dan Perkembangan Kalus Embriogenik dan Embrio Somatik Kelapa Sawit (Elaeis guineensis Jacq) pada Sistem Perendaman Sesaat. Menara Perkebunan. 75(1): 40.

[26] Suryowinoto, M. 1996. Prospek Kultur Jaringan dalam Perkembangan Pertanian Modern. Universitas Gadjah Mada. Yogyakarta.

[27] Utami, E.S.W., Sumardi, I., Taryono dan Semiarti, E. 2007. Pengaruh α-Naphtaleneacetic Acid (NAA) Terhadap Embriogenesis Somatik Anggrek Bulan Phalaenopsis amabilis (L.) Bl. Biodiversitas. 8(4): 295-296.

[28] Varga A, Thoma LH, Bruinsma J. 1988. Effect of auxin and cytokinins on epigenetic instability of callus propagated Kalanchoe blossfeldiana Poelln. Plan Cell Tissue and Org Culture. 15: 223-231

[29] Winarto, B. 2011. Pengaruh Glutamin dan Serin terhadap Kultur Anter Anthurium andraeanum cv. Tropical. J. Hort. 21(4): 295-296.

[30] Yuwono, T. 2006. Bioteknologi Pertanian. Universitas Gadjah Mada. Yogyakarta.

[31] Zulkarnain. 2009. Kultur Jaringan Tanaman. Bumi Aksara. Jakarta.